Pyrene degradation of biofilm-forming Paracoccus sp. DG25 isolated from oil polluted samples collected in petroleum storage Duc Giang, Hanoi

  • Thi Nhi Cong Le Department of Environmental Biotechnology, Institute of Biotechnology, Vietnam
  • Thi Ngoc Mai Cung Department of Environmental Biotechnology, Institute of Biotechnology, Vietnam
  • Thi Thanh Vu Department of Environmental Biotechnology, Institute of Biotechnology, Vietnam
  • Ngoc Minh Nghiem Genomic Research Institute, Vietnam Academy of Science and Technology; 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
  • Phuong Ha Hoang Department of Environmental Biotechnology, Institute of Biotechnology, Vietnam
  • Thi Lien Do Department of Environmental Biotechnology, Institute of Biotechnology, Vietnam
  • Thi To Uyen Do Department of Environmental Biotechnology, Institute of Biotechnology, Vietnam

Abstract

In this study, a well biofilm-forming bacterial strain was isolated from oil contaminated water and sediment samples collected in petroleum storage Duc Giang, Hanoi. It was identified as Paracoccus sp. DG25 and registered in the GenBank database with the accession numbers KJ608354. Several biophysical and bio-chemical conditions for the biofilm formation of the strain were estimated such as pH, temperature, carbon sources and nitrogen sources. As the results the biofilm forming capacity was highest at pH 7, 37 oC, on maltose and supplemented with KNO3. Using these optimal conditions, the formed biofilm degraded 76.07 % of pyrene after 7 day-incubation, with the initial concentration of 300 ppm by high-performance liquid chromatography (HPLC) analysis. To our knowledge, there is rare publication on pyrene degradation by biofilm-forming bacteria. Therefore, the obtained results show that biofilm formed the strain Paracoccus sp. DG25 may considerably increase the degrading efficiency of pyrene and may lead to a new approach to treat polycyclic aromatic hydrocarbons containing in petroleum oil contaminated water in Vietnam.

 

How to Cite
LE, Thi Nhi Cong et al. Pyrene degradation of biofilm-forming Paracoccus sp. DG25 isolated from oil polluted samples collected in petroleum storage Duc Giang, Hanoi. Journal of Vietnamese Environment, [S.l.], v. 6, n. 2, p. 178-183, sep. 2015. ISSN 2193-6471. Available at: <https://oa.slub-dresden.de/ejournals/jve/article/view/193>. Date accessed: 18 nov. 2017. doi: http://dx.doi.org/10.13141/jve.vol6.no2.pp178-183.
Section
Research articles

Keywords

biodegradation, biofilm, Paracoccus sp., pyrene